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Kirschner Lab Protocols
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Useful Links
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Useful Buffer/Protocol Pages
Kirschner Lab Buffers
Buffer page
on Reto Portmans web page
(
local copy
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Protocols-Online
Mass Spectrometry Protocols
In Gel Trypsin Digestion Procedure
Nucleic Acid Protocols
Fill In Reactioins using Klenow or T4 Polymerase
Gene Clean Procedure
MP Bio's Gene Clean II
Linearization of Plasmid DNA Procedure
Plasmid DNA Preparation using Wizard Kit
Promega's Wizard Miniprep kit
Dephosphorylating DNA with Alkaline Phosphatase
Ambion's mMessage mMachine
Novagen's pET system
alt link
PCDNA 3.1 Map
Qiagen Hi Speed Plasmid Kit
Qiagen Plasmid Purification
Qiagen Miniprep Kit
Qiagen Quick Manuals (PCR Purif., Nucleotide Removal, and Gel Extraction)
RNeasy MinElute Cleanup Handbook
Stratagene QuickChange Manual
X-Gal Staining
Embryo RT PCR
Protein Protocols
BioRad Criterion system Manual
BioRad Protein Assay
BioRad trans blot SD Manual
GE Healthcare ECL plus Manual
GE Healthcare AKTA Protein Purification Design Book
GE Healthcare Antibody Purification Book
GE Healthcare Gel Filtration Book
GE Healthcare Protein Electrophoresis Book
Invitrogen Bac to Bac Expression System
Invitrogen Library Efficiency DH5a Competent Cells
Invitrogen Max Efficiency DH5a Competent Cells
Invitrogen Novex Electrophoresis Guide
Millipore Centricon 70 (large volume spin)
Millipore Centricon Guide
Promega Dual Luciferase Manual
Promega Quick Coupled TNT Manual
Promega TNT Coupled Retic System
Promega TNT gold 96 System
Promega TNT SP6 High Yield System
Promega TNT Wheat Germ System
Qiagen's EasyXpress Kit
Qiagen's QIAexpressionist Kit