|
These Buffers and Media are used in the Protocols of our Lab |
| Index: | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z |
| Compound |
|
|
200 ml |
| Glycine | 75.07 |
|
1.5 g |
| 1 M MgCl2 |
|
2 ml | |
| KOH |
|
||
| H2O | to 200 ml |
| Compound |
|
|
50 ml |
| Acetic Acid 98% (~17 M) |
|
|
3 ml |
| H2O |
to 50 ml |
Acrylamide/Bisacrylamide
| Compound |
|
|
500 ml |
| Acrylamide |
|
|
100 g |
| Bisacrylamide |
|
|
4 g |
| H2O |
to 500 ml |
| Compound |
|
|
500 ml |
| Glucose |
|
|
4.5 g |
| Tris |
|
|
1.5 g |
| EDTA 0.25 M |
|
20 ml | |
| H2O |
to 450 ml | ||
| Adjust pH 8.0 with HCl | |||
| H2O, Autoclave, store at 4°C |
to 500 ml |
| Compound |
|
|
100 ml |
| Lysozyme |
|
0.02 g | |
| Alkaline Lysis Buffer A |
to 100 ml |
| Compound |
|
|
50 ml |
| SDS 20 % |
|
2.5 ml | |
| NaOH 10 M |
|
1 ml | |
| H2O |
to 50 ml |
| Compound |
|
|
50 ml |
| KAc |
|
|
14.7 g |
| Acetic Acid 100 % |
|
5.75 ml | |
| H2O |
to 50 ml |
| Compound |
|
|
100 ml |
| Amidoblack |
|
|
0.1 g |
| Acetic Acid 100 % |
|
7.5 ml | |
| Methanol |
|
20 ml | |
| H2O |
to 100 ml |
| Constituent |
|
|
ml stock for 1 liter medium |
| Adenine sulfate |
|
|
|
| Uracil |
|
|
|
| L-tryptophan |
|
|
|
| L-histidine-HCl |
|
|
|
| L-arginine-HCl |
|
|
|
| L-methionine |
|
|
|
| L-tyrosine |
|
|
|
| L-leucine |
|
|
|
| L-isoleucine |
|
|
|
| L-lysine-HCl |
|
|
|
| L-phenylalanine |
|
|
|
| L-glutamic acid |
|
|
|
| L-aspartic acid |
|
|
|
| L-valine |
|
|
|
| L-threonine |
|
|
|
| L-serine |
|
|
|
3-Aminophtalhydrazide 250 mM
| Compound |
|
|
10 ml |
| 3-Aminophtalhydrazide |
|
|
442 mg |
| H2O |
to 10 ml |
| Compound |
|
|
50 ml |
| Ammoniummolybdate x 4H2O |
|
|
2.1 g |
| H2O |
to 30 ml | ||
| HCl fuming 37 % (~12 M) |
|
16.7 ml | |
| H2O |
to 50 ml |
Ampicillin 20 mg/ml pH 8.0
| Compound |
|
|
15 ml |
| Ampicillin |
|
|
300 mg |
| H2O |
to 10 ml | ||
| Adjust pH 8.0 with NaOH | |||
| H2O, Filter sterilize |
to 15 ml |
Antibiotics
| Antibiotics |
|
|
Resistance Mechanism |
| Ampicillin |
|
Inhibits cell wall synthesis by inhibiting formation of the peptidoglycan crosslink | b-lactamase (286 aa) is secreted into the periplasm and cleaves lactam antibiotic |
| Chloramphenicol |
|
Inhibits protein synthesis by interacting with the 50S ribosomal subunit and inhibiting the peptidyltransferase reaction. | 216 aa protein cytoplasmic protein which acetylates chloramphenicol |
| Kanamycin Neomycin |
|
Inhibits translocation and elicits miscoding by binding to the 30S ribosomal subunit. | 256 aa aminoglycoside phosphotransferase phosphorylates kanamycin, neomycin, etc. |
| Tetracycline |
|
Inhibits protein synthesis by preventing binding of aminoacyl tRNA to the ribosome A site. | 428 aa membrane protein catalyzing efflux of tetracyclin, probably by a cation symport mechanism. |
APS
| Compound |
|
|
10 ml |
| Ammoniumpersulfate |
|
|
1 g |
| H2O, store at 4°C |
to 10 ml |
Ascorbate 0.5 M pH 6.0
| Compound |
|
|
50 ml |
| L-Ascorbic acid |
|
|
4.4 g |
| H2O |
to 22 ml | ||
| Adjust pH 6.0 with NaOH 1 M | |||
| H2O |
to 50 ml |
Asolectin (fatty acid free) 50 mg/ml
| Compound |
|
|
50 ml |
| Asolectin (aceton treated) |
|
|
2.5 g |
| Diethylether, store at -20°C |
to 50 ml |
ATP 200 mM
| Compound |
|
|
10 ml |
| Adenosine-5'-Triphosphate |
|
|
1.102 g |
| H2O |
to 5 ml | ||
| Adjust pH 6.5 with NaOH 1 M | |||
| H2O, store at -20°C |
to 10 ml |
ATP Regenerating System (ARS) 40x
| Compound |
|
|
1 ml |
| Phospoenolpyruvate (PEP) |
|
|
4.16 mg |
| Pyruvate Kinase (PK) 5 U/ml |
|
20 ml | |
| H2O, store at 4°C |
to 1 ml |
| Compound |
|
|
10 ml |
| Aprotinin 1.5 mg/ml |
|
3.24 ml | |
| PepstatinA 1 mg/ml in DMSO |
|
|
1.3 ml |
| Leupeptin x 1/2H2SO4 10.5 mM |
|
|
37.5 ml |
| 4-Aminobenzamidine x 2HCl |
|
|
396 mg |
| H2O |
to 10 ml |
Bis-Tris 1M pH7.0
| Compound |
|
|
100 ml |
| Bis-Tris |
|
|
20.9 g |
| H2O |
to 50 ml | ||
| Adjust pH 7.0 with HCl | |||
| H2O |
to 100 ml |
Brij/Doc pH 8.0
| Compound |
|
|
100 ml |
| EDTA 0.25 M |
|
|
0.4 ml |
| Tris 1 M pH 8.0 |
|
1 ml | |
| Na-Deoxycholate 8 % |
|
5 ml | |
| Brij 58 |
|
1 g | |
| H2O |
to 90 ml | ||
| Dissolve at 37°C, adjust pH 8.0 | |||
| H2O, Filter sterilize | to 100 ml |
BS3 Buffer pH7.5
| Compound |
|
|
1000 ml |
| HEPES |
|
|
23.83 g |
| Dodecylmaltoside |
|
|
0.5 g |
| EDTA 0.25 M |
|
4 ml | |
| DTT |
|
|
1.54 g |
| H2O |
to 900 ml | ||
| Adjust pH 7.5 with NaOH 10M | |||
| H2O | to 1000 ml |
| Compound |
|
|
1.5 ml |
| CdSO4 |
|
|
313 mg |
| H2O |
to 1.5 ml |
Chloroform
| Compound |
|
|
50 ml |
| Chloroform |
|
48 ml | |
| Isoamyl alcohol |
|
2 ml |
| Compound |
|
|
15 ml |
| EDTA 0.25 M pH 8.0 |
|
|
12 ml |
| Tris 1 M pH 8.0 |
|
6 ml | |
| H2O |
3 ml |
Citrate 1M pH5.O
| Compound |
|
|
50 ml |
| Citrate x H2O |
|
|
10.5 g |
| H2O |
to 35 ml | ||
| Adjust pH 5.0 with NaOH 10M | |||
| H2O | to 50 ml |
Colony PCR Buffer 10x
| Compound |
|
|
15 ml |
| Tris 1M pH6.8 |
|
|
1.875 ml |
| KCl |
|
|
0.629 g |
| H2O, Autoclave |
to 15 ml |
Coomassie Blue Solution
| Compound |
|
|
1000 ml |
| Coomassie Blue (Serva Blau) |
|
|
2 g |
| Acetic acid |
|
75 ml | |
| Methanol |
|
500 ml | |
| H2O |
to 1000 ml |
Coumaric Acid 40 mM
| Compound |
|
|
10 ml |
| p-Coumaric acid |
|
|
66 mg |
| H2O |
to 10 ml |
CS/PBS
| Compound |
|
|
200 ml |
| PBS, 20x |
|
|
10 ml |
| Newborn calf serum |
|
20 ml | |
| H2O, Store frozen | 200 ml |
CuSO4 1 M
| Compound |
|
|
25 ml |
| CuSO4 |
|
|
4 g |
| H2O |
to 25 ml |
| Compound |
|
|
1000 ml |
| H2O |
|
1000 ml | |
| Diethylpyrocarbonate (DECP) |
|
1 ml | |
| Let sit at RT for 1 h, Autoclave |
Destain Solution
| Compound |
|
|
1000 ml |
| Acetic acid |
|
75 ml | |
| Ethanol |
|
100 ml | |
| H2O |
to 1000 ml |
Developer 5x w/o
| Compound |
|
|
2000 ml |
| Na2CO3 |
|
200 g | |
| H2O |
to 2000 ml |
| Product/Reagent | Volume |
|
| Visking 20/32 tubing |
|
|
| 2 % NaHCO3 |
|
|
| 10 mM EDTA |
|
|
| 10 mM EDTA |
|
|
| H2O |
|
DNA Loading Buffer 6x
| Compound |
|
|
10 ml |
| Bromophenolblue |
|
|
25 mg |
| Xylene Xyanol |
|
25 mg | |
| Sucrose |
|
4 g | |
| H2O |
to 10 ml |
Dodecylmaltoside 10 %
| Compound |
|
|
50 ml |
| Dodecylmaltoside |
|
|
5 g |
| H2O, Store at 4°C |
to 50 ml |
DTT 1 M
| Compound |
|
|
5 ml |
| Dithiothreitol |
|
|
770 mg |
| H2O, Store at 4°C |
to 10 ml |
| Compound |
|
|
102 ml |
| Tris-Cl 200 mM pH 8.5 |
|
|
100 ml |
| 3-Aminophtalhydrazine 250 mM |
|
1 ml | |
| Coumaric acid 40 mM, Filter |
|
1 ml |
ECL Solution 2
| Compound |
|
|
100 ml |
| H2O |
|
|
100 ml |
| H2O2 30 % |
|
60 ml |
EDTA (Na-Salt) 0.25 M pH8.0
| Compound |
|
|
100 ml |
| EDTA (Na2 salt) |
|
|
9.3 g |
| H2O |
to 70 ml | ||
| Adjust pH 8.0 with 10 M NaOH | |||
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
1.5 ml |
| FeCl3 x 6 H2O |
|
|
406 mg |
| H2O |
to 1.5 ml |
Ferrozine 100 mM
| Compound |
|
|
1 ml |
| Ferrozine |
|
|
49.2 ml |
| NaOH 1 M |
2 ml | ||
| H2O |
|
to 1 ml |
Folin 50 %
| Compound |
|
|
50 ml |
| Folin Reagens |
|
|
25 ml |
| H2O |
|
to 50 ml |
FPLC Solution A pH 7.4
| Compound |
|
|
500 ml |
| Tris |
|
|
3.03 g |
| Glycerol 87 % |
|
57.5 ml | |
| Dodecylmaltoside 10 % |
|
5 ml | |
| H2O |
to 460 ml | ||
| Adjust pH 7.4 with 1 M H2SO4 | |||
| H2O |
to 500 ml |
FPLC Solution B pH 7.4
| Compound |
|
|
500 ml |
| Imidazol |
|
|
6.8 g |
| Tris |
|
|
3.03 g |
| Glycerol 87 % |
|
57.5 ml | |
| Dodecylmaltoside 10 % |
|
5 ml | |
| H2O |
to 460 ml | ||
| Adjust pH 7.4 with 1 M H2SO4 | |||
| H2O |
to 500 ml |
FPLC Solution C pH 7.4
| Compound |
|
|
500 ml |
| K2SO4 |
|
|
17.4 g |
| Tris |
|
|
3.03 g |
| Glycerol 87 % |
|
57.5 ml | |
| Dodecylmaltoside 10 % |
|
5 ml | |
| H2O |
to 460 ml | ||
| Adjust pH 7.4 with 1 M H2SO4 | |||
| H2O |
to 500 ml |
French Press Buffer (FPB)
| Compound |
|
|
500 ml |
| Tris-SO4 1 M pH 7.4 |
|
|
25 ml |
| Glycerol 87 % |
|
57.5 ml | |
| H2O, Autoclave |
to 500 ml |
| Compound |
|
|
1.5 ml |
| Glutathione (GSH) |
|
|
46 mg |
| H2O, Store at 4°C |
to 1.5 ml |
Glycerol 50 %
| Compound |
|
|
50 ml |
| Glycerol 87 % |
|
28.75 ml | |
| H2O, Autoclave |
to 50 ml |
| Compound |
|
|
50 ml |
| HCl 37 % (~12M) |
|
|
4.2 ml |
| H2O |
to 50 ml |
HIS-Stock 2 mg/ml
| Compound |
|
|
100 ml |
| L-Histidine |
|
|
200 mg |
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
5 ml |
| Isopropyl-b,D-thiogalactoside |
|
|
1.19 g |
| H2O, Filter sterilize |
to 5 ml |
| Compound |
|
|
15 ml |
| Kanamycin | 582.6 |
|
150 mg |
| H2O, Filter sterilize |
|
to 15 ml |
KH2PO4 0.4 M
| Compound |
|
|
50 ml |
| KH2PO4 | 136.1 |
|
2722 mg |
| H2O |
|
to 50 ml |
K-Na-Tartrate 2 %
| Compound |
|
|
50 ml |
| K-Na-tartrate x 4 H2O |
|
|
1 g |
| H2O |
to 50 ml |
| Compound |
|
|
10 liters |
| Tris |
|
|
30 g |
| Glycine |
|
|
144 g |
| EDTA (Triplex II) |
|
|
2.9 g |
| SDS |
|
|
10 g |
| H2O |
|
to 10 liters |
LB Media
| Compound |
|
|
1000 ml |
| Yeast Extract (BBL) |
|
5 g | |
| Trypticase Peptone (BBL) |
|
10 g | |
| NaCl |
|
10 g | |
| H2O, Autoclave |
|
to 1000 ml |
| Compound |
|
|
1000 ml |
| Yeast Extract (BBL) |
|
5 g | |
| Trypitcase Peptone (BBL) |
|
10 g | |
| NaCl |
|
10 g | |
| Agar |
|
15 g | |
| H2O, Autoclave |
|
to 1000 ml |
LB Low Salt Liquid
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
5 g | |
| Bacto Peptone |
|
10 g | |
| NaCl |
|
5 g | |
| H2O, Autoclave |
|
to 1000 ml |
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
5 g | |
| Bacto Peptone |
|
10 g | |
| NaCl |
|
5 g | |
| Agar |
|
15 g | |
| H2O, Autoclave |
|
to 1000 ml |
LEU-Stock 3 mg/ml
| Compound |
|
|
50 ml |
| L-Leucine |
|
150 mg | |
| H2O, Filter sterilize |
|
to 50 ml |
LiAc 1 M
| Compound |
|
|
100 ml |
| LiAc-dihydrate |
|
|
10.2 g |
| H2O |
to 60 ml | ||
| TE 10x |
10 ml | ||
| H2O, Autoclave |
to 100 ml |
LiCl 1 M
| Compound |
|
|
50 ml |
| LiCl |
|
|
2.12 g |
| H2O, Autoclave |
to 50 ml |
Lowry Component
| Compound |
|
|
200 ml |
| Na2CO3 |
|
|
6 g |
| NaOH | 40 |
|
800 mg |
| H2O |
|
to 200 ml |
LYS-Stock 3 mg/ml
| Compound |
|
|
50 ml |
| DL-Lysine |
|
|
150 mg |
| H2O, Autoclave |
|
to 50 ml |
| Compound |
|
|
1000 ml |
| Neopeptone Difco |
|
|
5 g |
| Bacto-Soytone |
|
5 g | |
| Beef Extract |
|
5 g | |
| Yeast Extract |
|
2.5 g | |
| Ascorbic Acid |
|
|
0.5 g |
| 2-Glycerolphosphate x 5 H2O, disodium
salt Note: we used 19 g/l up to Dec. 2002 |
|
|
1 g |
| H2O, Autoclave, Chill to RT |
to 1000 ml | ||
| Glucose 50 % |
|
|
20 ml |
| MgSO4 1 M |
|
1 ml |
| Compound |
|
|
50 ml |
| Malachit Green |
|
|
30 mg |
| H2O |
to 50 ml |
MES 100 mM pH 6.4
| Compound |
|
|
250 ml |
| MES |
|
|
4.88 g |
| H2O |
to 200 ml | ||
| Adjust pH 6.4 with NaOH | |||
| H2O, Autoclave |
to 250 ml |
| Compound |
|
|
15 ml |
| MgSO4 |
|
|
3.70 g |
| H2O |
to 15 ml |
| Compound | MW | Final concentration | 1000ml |
| Trypticase Peptone (BBL) | 1 % | 10 g | |
| Beef Extract (Difco) | 0.3 % | 3 g | |
| Yeast Extract | 0.5 % | 5g | |
| K2HPO4 | 174 | 11.5 mM | 2 g |
| NH4Acetate | 77 | 39 mM | 3 g |
| tri-Na-Citrate x 2H2O | 294 | 10.2 mM | 3 g |
| MgSO4 1 M | 246 | 0.8 mM | 0.8 ml |
| MnSO4 0.4 M | 0.4 mM | 1 ml | |
| Tween 80 | 0.1 % | 1 ml | |
| Adjust pH 6.3 with 5N HCl | |||
| H2O, Autoclave | to 1000 ml | ||
| Glucose 50% (or Lactose = MRSL) |
1 % | 20 ml |
| Compound |
|
|
50 ml |
| NaAc x 3H2O |
|
|
17 g |
| H2O |
to 35 ml | ||
| Adjust pH 5.2 with Acetic Acid |
ca. 10 ml | ||
| H2O, Autoclave |
to 50 ml |
Na-Citrate 34 %
| Compound |
|
|
50 ml |
| Na-citrate x 2H2O |
|
|
17 g |
| H2O |
to 50 ml |
NaCl 5 M
| Compound |
|
|
50 ml |
| NaCl |
|
|
14.6 g |
| H2O |
to 50 ml |
Na-Deoxycholate 8%
| Compound |
|
|
30 ml |
| Deoxiycholic acid (Na salt) |
|
4 g | |
| H2O |
to 20 ml | ||
| Adjust pH 8.4 with 10 M NaOH | |||
| H2O, Filter sterilize |
to 30 ml |
NaOH 10M
| Compound |
|
|
500 ml |
| NaOH |
|
|
200 g |
| H2O |
to 500 ml |
Nick Translation Buffer 4x
| Compound |
|
|
10 ml |
| Tris-Cl 1 M pH 7.2 |
|
2 ml | |
| MgSO4 1 M |
|
400 ml | |
| DTT 1 M |
|
4 ml | |
| H2O |
to 10 ml |
NiSO4 1M
| Compound |
|
|
1.5 ml |
| NiSO4 |
|
|
232 mg |
| H2O |
to 1.5 ml |
N-Media
| Compound |
|
|
500 ml |
| Na2HPO4 x 2H2O |
|
|
5.33 g |
| Bacto Yeast Extract |
|
2.5 g | |
| Bacto Peptone |
|
5 g | |
| H2O, Autoclave |
to 490 ml | ||
| Glucose 50 % |
|
10 ml |
NTA Regeneration Buffer
| Compound |
|
|
15 ml |
| Guanidine-HCl (GuHCL) |
|
|
8.6 g |
| Acetic Acid 98 % (~17 M) |
|
175 ml | |
| H2O |
to 15 ml |
| Compound |
|
|
10 ml |
| Nitrotetrazolium blue |
|
200 mg | |
| Dimethylsulfoxide |
5 ml | ||
| H2O | to 10 ml |
NTP-Mix 12.5 mM each
| Compound |
|
|
80 ml |
| dATP 100 mM |
|
|
10 ml |
| dGTP 100 mM |
|
10 ml | |
| dCTP 100 mM |
|
10 ml | |
| dTTP 100 mM |
|
10 ml | |
| H2O |
40 ml |
| Compound |
|
|
500 ml |
| K2Cr2O7 |
|
|
5 g |
| H3PO4 |
|
0.5 ml | |
| H2O |
to 500 ml |
| Compound |
|
|
1000 ml |
| Na2HPO4 x 2H2O |
|
|
28.5 g |
| NaH2PO4 x H2O |
|
|
5.52 g |
| NaCl |
|
|
164 g |
| H2O (pH should be 7) |
to 1000 ml |
PBS au lait
| Compound |
|
|
300 ml |
| PBS 20x |
|
|
15 ml |
| Defatted powdered milk (Sano Lait, Coop) |
|
6 g | |
| Azide, 20% stock (to stabilize; inhibits lactoperoxidase!) |
|
0.3 ml | |
| H2O |
to 300 ml |
PBS-Tween
| Compound |
|
|
300 ml |
| PBS 20x |
|
|
15 ml |
| Tween 20 |
|
600 ml | |
| H2O |
to 300 ml |
PEG4000 50%
| Compound |
|
|
100 ml |
| Polyethylene glycol 4000 |
|
50 g | |
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
50 ml |
| Phenol, distilled |
|
50 ml | |
| TE |
|
~20 ml | |
| 8-Hydroxyquinoline |
|
50 mg | |
| Mercaptoethanol |
|
100 ml |
Phenol (stabilized)
| Compound |
|
|
50 ml |
| Phenol (stabilized) |
|
50 ml | |
| Mercaptoethanol |
|
100 ml |
| Compound |
|
|
50 ml |
| Phenol |
|
25 ml | |
| Chloroform |
|
24 ml | |
| Isoamyl alcohol |
|
1 ml |
PIM Protease Inhibitor Mix
| Compound |
|
|
20 ml |
| TLCK (Tosyl-Lysinechloromethylketone) | 369 |
|
738 mg |
| TPCK (Tosyl-Phenylalaninechloromethylketone) |
|
|
704 mg |
| p-Aminobenzamidin-HCl | 208 |
|
416 mg |
| o-Phenanthroline | 198 |
|
396 mg |
| PMSF (Phenylmethylsulfonylfluoride) | 174 |
|
348 mg |
| DMSO, Store 2 ml aliquots at -20°C (keeps up to 1 year or longer) |
|
to 20 ml |
PIS Protease Inhibitor Mix
| Compound |
|
|
20 ml |
| TLCK (Tosyl-Lysinechloromethylketone) | 369 |
|
738 mg |
| TPCK (Tosyl-Phenylalaninechloromethylketone) |
|
|
704 mg |
| p-Aminobenzamidin-HCl | 208 |
|
416 mg |
| PMSF (Phenylmethylsulfonylfluoride) | 174 |
|
348 mg |
| DMSO, Store 2 ml aliquots at -20°C (keeps up to 1 year or longer) |
|
to 20 ml |
PLATE
| Compound |
|
|
50 ml |
| PEG4000 |
|
20 g | |
| LiAc |
|
|
0.51 g |
| Tris-Cl 1 M pH 7.5 |
|
500 ml | |
| EDTA 0.25 M |
|
200 ml | |
| H2O, Autoclave |
|
to 50 ml |
Poly-DL-Lysine
| Compound |
|
|
5 ml |
| Poly-DL-Lysine |
|
50 mg | |
| H2O, store at -20°C |
|
to 5 ml |
Protein Cracking Buffer
| Compound |
|
|
50 ml |
| Tris 1M pH 7.5 |
|
1.25 ml | |
| NaCl | 58.4 |
|
0.44 g |
| Dithiothreitol | 154 |
|
8 mg |
| H2O, Store at 4°C |
|
to 50 ml |
Protein Running Gel Buffer 1/2
| Compound |
|
|
500 ml |
| Tris 1M pH 8.8 |
|
375 ml | |
| SDS 20% |
|
5 ml | |
| EDTA 0.25M |
|
8 ml | |
| H2O |
|
to 500 ml |
Protein Sample Buffer 3x
| Compound |
|
|
20 ml |
| Tris 0.1M pH 8 |
|
6 ml | |
| Glycerol 50% |
|
|
12 ml |
| SDS |
|
1.5 g | |
| DTT (Dithiothreitol) |
|
30 mg | |
| Bromphenol blue |
|
10 mg | |
| H2O |
|
to 20 ml |
Protein Stacking Gel Buffer 1/3
| Compound |
|
|
500 ml |
| Tris 1M pH 6.8 |
|
83 ml | |
| SDS 20% |
|
3.3 ml | |
| EDTA 0.25M |
|
5.3 ml | |
| H2O |
|
to 500 ml |
PS 5x
| Compound |
|
|
1000 ml |
| Na2HPO4 x 2H2O |
|
|
71.2 g |
| NaH2PO4 x H2O |
|
|
13.8 g |
| NaCl |
|
|
29.25 g |
| H2O |
to 1000 ml |
PS-Tween
| Compound |
|
|
300 ml |
| PBS 5x |
|
|
60 ml |
| Tween 20 |
|
600 ml | |
| H2O |
to 300 ml |
PS-Tween au lait
| Compound |
|
|
15 ml |
| Defatted powdered milk (Sano Lait, Coop) |
|
1.5 g | |
| PS-Tween |
to 15 ml |
| Compound |
|
|
500 ml |
| Tris |
|
|
3.03 g |
| H2O |
|
to 450 ml | |
| Adjust pH 7.5 with 1 M H2SO4 | |||
| H2O |
|
to 500 ml |
Rusconi Mix
| Compound |
|
|
10 ml |
| Lysozyme 10 mg/ml |
|
500 ml | |
| EDTA 0.25M |
|
1 ml | |
| Tris 1M pH 7.5 |
|
250 ml | |
| RNase A 10 mg/ml |
|
100 ml | |
| DNA Loading Buffer 6 x |
|
1.5 ml | |
| H2O |
|
to 10 ml |
| Compound |
|
|
1000 ml |
| Yeast Nitr. Base w/o aa |
|
6.7 g | |
| Dextrose |
|
20 g | |
| H2O, Autoclave |
to 1000 ml |
| Compound |
|
|
1000 ml |
| Yeast Nitr. Base w/o aa |
|
6.7 g | |
| Dextrose |
|
20 g | |
| Agar |
|
20 g | |
| H2O, Autoclave, cool, pour |
to 1000 ml |
| Compound |
|
|
1000 ml |
| AgNO3 |
|
|
20 g |
| H2O, store in a brown bottle |
to 1000 ml |
| Compound |
|
|
250 ml |
| Sorbitol |
|
36.43 g | |
| H2O, Autoclave |
to 250 ml |
SSC 20x
| Compound |
|
|
1000 ml |
| NaCl |
|
|
175 g |
| Na3-citrate |
|
|
88.2 g |
| H2O |
to 450 ml | ||
| Adjust pH 7.0 with 1 M HCl | |||
| H2O |
|
to 500 ml |
STET
| Compound |
|
|
50 ml |
| Sucrose (Saccharose) |
|
4 g | |
| Triton X-100 |
|
2.5 ml | |
| EDTA 0.25 M |
|
200 ml | |
| Tris-Cl 1 M pH 8.0 |
|
2.5 ml | |
| Na-azide 20 % |
|
50 ml | |
| H2O |
|
to 50 ml |
Sucrose 25 % in Tris pH8.0
| Compound |
|
|
100 ml |
| Sucrose |
|
|
25 g |
| Tris 1 M pH 8.0 |
|
5 ml | |
| H2O, Autoclave |
to 100 ml |
Sulfuric Acid 1 M
| Compound |
|
|
50 ml |
| H2O |
|
|
40 ml |
| H2SO4 95-97 % (~18 M) |
|
2.8 ml | |
| H2O |
to 50 ml |
| Compound |
|
|
100 ml |
| Tris-base 1 M |
|
1 ml | |
| EDTA 0.25 M |
|
0.4 ml | |
| Adjust pH 8.0 with HCl | |||
| H2O, Autoclave | to 100 ml |
TE 10x
| Compound |
|
|
100 ml |
| Tris-Cl 1 M pH 8.0 |
|
10 ml | |
| EDTA 0.25 M |
|
4 ml | |
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
500 ml |
| EDTA (Na2 salt) |
|
|
18.6 g |
| Tris |
|
|
242 g |
| H2O |
to 400 ml | ||
| Acetic acid 100 % |
|
57 ml | |
| H2O |
to 500 ml |
TES
| Compound |
|
|
100 ml |
| Tris 1 M pH 8.0 |
|
5 ml | |
| EDTA 0.25 M |
|
2 ml | |
| NaCl |
|
|
0.3 g |
| H2O, Autoclave |
to 100 ml |
TFB1
| Compound |
|
|
500 ml |
| KAc |
|
|
1.473 g |
| RbCl |
|
|
605 mg |
| CaCl2 x 2H2O |
|
|
735 mg |
| MnCl2 |
|
|
3.145 g |
| Glycerol 87 % |
|
86 ml | |
| H2O |
to 450 ml | ||
| Adjust pH 5.8 with 0.2 M AcOH | |||
| H2O, Filter sterilize |
to 500 ml |
| Compound |
|
|
500 ml |
| MOPS |
|
|
1.047 g |
| RbCl |
|
|
605 mg |
| CaCl2 x 2H2O |
|
|
5.51 g |
| MnCl2 |
|
|
3.145 g |
| Glycerol 87 % |
|
86 ml | |
| H2O |
to 450 ml | ||
| Adjust pH 6.5 with 0.2 M KOH | |||
| H2O, Filter sterilize |
to 500 ml |
Tricine Anode Buffer (+)
| Compound |
|
|
1000 ml |
| Tris |
|
|
24.23 g |
| H2O |
to 900 ml | ||
| Adjust pH 8.9 with HCl | |||
| H2O |
to 1000 ml |
Tricine Cathode Buffer (-)
| Compound |
|
|
1000 ml |
| Tris |
|
|
12.11 g |
| Tricine |
|
|
17.92 g |
| SDS 20% |
|
5 ml | |
| H2O | to 1000 ml |
Tricine Gel Acrylamide
| Compound |
|
|
250 ml |
| Acrylamide |
|
|
120 g |
| Methylene-Bisacrylamide |
|
|
3.75 g |
| H2O, avoid sunlight | to 250 ml |
Tricine Gel Buffer
| Compound |
|
|
250 ml |
| Tris |
|
|
90.86 g |
| H2O |
to 200 ml | ||
| Adjust pH 8.45 with HCl | |||
| SDS 20% |
|
3.75 ml | |
| H2O | to 250 ml |
Tris 1 M pH X
| Compound |
|
|
100 ml |
| Tris |
|
|
12.1 g |
| H2O |
to 80 ml | ||
| Adjust pH X with HCl | |||
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
100 ml |
| Tris |
|
|
12.1 g |
| H2O |
to 90 ml | ||
| Adjust pH 7.4 with H2SO4 | |||
| H2O, Autoclave |
to 100 ml |
TRP-Stock 2 mg/ml
| Compound |
|
|
40 ml |
| L-Tryptophan |
|
|
80 mg |
| H2O, Filter sterilize |
to 40 ml |
TSS
| Compound |
|
|
50 ml |
| Polyethylene glycol 6000 | 6000 |
|
5 g |
| DMSO |
|
2.5 ml | |
| MgSO4 |
|
|
1 ml |
| LB, Autoclave |
to 50 ml |
| Compound |
|
|
100 ml |
| Uracil |
|
|
200 mg |
| H2O, Autoclave |
to 100 ml |
| Compound |
|
|
5 ml |
| Na3VO4 x 5 H2O |
|
|
27.4 mg |
| H2O, store at -20°C |
|
to 5 ml |
| Compound |
|
|
2000 ml |
| Tris |
|
|
72.7 g |
| Glycine |
|
|
300 g |
| SDS 20% |
|
|
4 g |
| H2O |
|
to 2000 ml |
| Compound |
|
|
10 ml |
| 5-Bromo-4-chloro-indoxylphosphate |
|
100 mg | |
| Dimethylsulfoxide |
to 10 ml |
| Compound |
|
|
50 ml |
| Triton X-100 |
|
1 ml | |
| SDS 20 % |
|
2.5 ml | |
| NaCl |
|
|
0.3 g |
| Tris-Cl 1 M pH 8.0 |
|
500 ml | |
| EDTA 0.25 M |
|
200 ml | |
| H2O |
to 50 ml |
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
10 g | |
| Bacto Peptone |
|
20 g | |
| Dextrose |
|
20 g | |
| H2O, Autoclave |
to 1000 ml |
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
10 g | |
| Bacto Peptone |
|
20 g | |
| Dextrose |
|
20 g | |
| Agar |
|
20 g | |
| H2O, Autoclave, cool, pour |
to 1000 ml |
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
10 g | |
| Bacto Peptone |
|
20 g | |
| Galactose |
|
20 g | |
| H2O, Autoclave |
to 1000 ml |
| Compound |
|
|
1000 ml |
| Bacto Yeast Extract |
|
10 g | |
| Bacto Peptone |
|
20 g | |
| Galactose |
|
20 g | |
| Agar |
|
20 g | |
| H2O, Autoclave, cool, pour |
to 1000 ml |
| Compound |
|
|
1.5 ml |
| ZnSO4 x 7 H2O |
|
|
431 mg |
| H2O |
to 1.5 ml |
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Last updated: June 24, 2004. Address comments to the Webmaster
.
