Kirschner Lab Buffers

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10x PBS          100 mL            pH 9.2

8 g       NaCl

0.2 g    KCl

1.15 g Na2HPO4

0.2 g    KH2PO4

 

pH to 9.2 with NaOH

 

50 mM Sodium Bicarbonate

0.042 g in 10 mL DI H2O

 

pH to 9.5 with NaOH

 

Rhodamine-B                        (5 mg / mL      5x     pH 9.7-10)

0.025 g in 5 mL 50 mM Sodium Bicarbonate (pH 9.7 – 10)

 

Store at 4C wrapped in foil

 

50 mM Sodium Bicarb Buffered Saline (BBS)       1 L

Stock A: 0.1 M NaHCO3 (fw 84.01)

            8.4 g à 1 L

or

            150 mL of 750 mM NaHCO3 à 1125 mL (pH 8.4)

 

Stock B: 0.1 M Na2CO3 (fw 106)

            10.6 g à 1 L

 

BBS pH 8.5 (1 L)

            500 mL            Stock A

            ~2 mL                         Stock B (pH to 8.5)

            8.5 g                NaCl

            DI H2O to 1 L

 

BBS pH 9.3 (1 L)

            400 mL            Stock A

            ~100 mL         Stock B (pH to 9.3)

            8.5 g                NaCl

            DI H2O to 1 L

 

2 M NH4Cl    (10 mL)

1.06 g to 10 mL DI H2O

Filter

 

 

 

 

Prehybridization mix (40 mL)

1 % BSA                                 10 mL 4 % BSA

1 % SDS                                 2 mL    20 % SDS

0.5 M NaPO4 (pH 7.9)           20 mL 1 M NaPO4 (ph 7.9)

1 mM EDTA                           400 uL 100 mM EDTA (pH 8)

100 ug / mL carrier DNA        400 uL 10 mg / mL salmon sperm DNA

                                                            7.2 mL DI H2O

 

Store frozen (-20C)

 

Hybridization mix

1 vol                kinase reaction

100 vol            prehybridization mix

 

Acrylamide / Bis (30 % / 0.8 %) (200 mL)

60 g     electrophoresis grade acrylamide

1.65 g bis (electrophoresis grade)

DI H2O to 200 mL

 

Stir

Store at 4C wrapped in foil

 

Use with 10 % (wt/vol) persulfate made fresh

0.1  g persulfate in 1 mL DI H2O

 

Laemmli Sample Buffer (10 mL 5X)

2.5 mL             1.25 M Tris (pH 6.8)

5 mL                100 % glycerol

1 g                   SDS (electrophoresis grade)

0.8 g                DTT (electrophoresis grade)

1 mL                Bromophenol Blue (1 % solution)

 

DI H2O to 10 mL (5X)

 

 

 

 

 

 

 

 

 

 

 

 

 

Laemmli Gel Buffers

Buffer I (8X     3 M     pH 8.8     200 mL)

18 g     Tris.HCl

58.8 g Tris Base

DI H2O to 200 mL

 

pH to 8.8

 

Buffer II (8X     1M     pH 6.8     100 mL)

14.4 g Tris.HCl

1.3 g    Tris Base

DI H2O to 100 mL

pH to 6.8

 

Laemmli Running Buffer (5X     1 L)

15.14 g            Tris (mw 121.1)

72.1 g              Glycine (mw 75.1)

5 g                   Lauryl Sulfate

DI H2O to 1 L

 

Store at 4C

 

HO Buffer without BME

250 mM           NaCl

10 mM             Tris (pH 7.4)

 

Fluorescein Working Stock (10 mg / mL     10X     pH 9.7 - 10)

10 mg / mL      fluorescein in

50 mM Sodium bicarbonate pH 9.7 - 10

 

Alcoholic KOH for plate washing

In 500 mL beaker, cover bottom with KOH pellets

~150 mL         DI H2O to dissolve KOH

~250 mL         ETOH (95 %)

 

Dialysis Buffer (1 L)

150 mM           NaCl                            8.8 g

15 mM             MgCl                           15 mL 1M MgCl   (or 3.04 g)

10 mM             Tris (pH 7.4)                10 mL 1 M Tris (pH 7.4)

                                    DI H2O                       to 1 L

 

 

 

 

 

 

Stabilization Buffer for Microtubules

Stock A: 1 M PIPES (pH ~ 7.3)

33.5 g  PIPES in 100 mL DI H2O

 

Stock B: 0.1 M EGTA (pH ~ 6.9)

            1.9 g    EGTA

            15 mL  DI H2O

           

            pH with 1 M KOH

            add DI H2O to 50 mL

 

Stabilization Buffer (10 mL)

            1 mL                Stock A           (0.1 M PIPES)

            0.1 mL             Stock B           (1 mM EGTA)

            0.4 g                PEG 8000       (4 % Polyethylene Glycol)

            13 mg              GTP (Na salt)  (2.5 mM GTP)

            +/- 5 uL / mL   Triton X-100   (0.5 % Triton X-100)

 

PM2G Buffer (pH 6.9     1 L)

            0.76 g              EGTA             (2 mM)

            0.246 g            MgSO4.7H2O            (1 mM)

            33.35 g            PIPES             (100 mM)

            400 mL            DI H2O

184.2 g            Glycerol          (2 M)

 

            pH to 6.9 with 10 N NaOH

            add DI H2O to 1 L

            + 0.1 % NP40 = 20 uL / mL (5 % NP40 stock)

            + PMSF = 1 uL / mL 0.2 M PMSF stock

 

PM Buffer (pH 6.9     100 mL)

            3.34 g              PIPES             (100 mM)

            0.025 g            MgSO4.7H2O            (1 mM)

            1.47 g              CaCl2              (1 mM)

            50 mL              DI H20

 

            pH to 6.9 with 10 N NaOH

            add DI H2O to 100 mL

 

GSD Buffer (6 mL     4X)

            2 mL                Glycerol

            4 mL                10 % SDS

            0.3 g                DTT

            Some               Bromophenol Blue

           

            Add DI H2O to 6 mL

 

TNT Buffer (6 L)

            75 mL              2M Tris (pH 7.5)

            59 g                 NaCl

            30 mL              Tween 20 (0.5 %)

 

            Add DI H2O to 6 L

 

2 M Tris (pH 7.5     500 mL)

            121 g               Tris Base

            300 mL            DI H2O

           

            pH with (lots) of HCl

            Add DI H2O to 500 mL

 

PMSF (0.2 M     1000X     1 mL)

            34.8 mg / mL in 95 % EtOH

            Phenyl methyl sulfonyl fluoride (mw 174.2)

 

1 M Sodium Phosphate for blotting

            1 L                   1 M Di-basic Na2H(PO4)

            500 mL            1 M Mono-basic NaH2(PO4)

           

            Dilute to 20 mM for transfer

 

CsCl (density gradient)

            74.2 g / 100 mL           CsCl    (1.546 g / mL)

            37.1 g / 50 mL             CsCl    (1.546 g / mL)

           

            61 g / 100 mL              CsCl    (1.45 g / mL)

 

            27.7 g / 100 mL           CsCl    (1.2 g / mL)

 

            Use DI H2O or Dialysis Buffer

 

Elisa III Buffer

15 mL              1 M NaCl                    (150 mM NaCl)

1 mL                100 mM EDTA           (1 mM EDTA)

5 mL                1 M Tris (pH 7.4)        (50 mM Tris.HCl)

50 uL               Tween 20                    (0.05 % Tween 20)

 

Adjust pH to 7.4

Add DI H2O to 100 mL

Add BSA to 0.1 %

 

 

1 M MOPS (pH 7.5     400 mL)

            83.72 g            MOPS

            250 mL            DI H2O

            pH to 7.5 with 10 N NaOH

            Add DI H2O to 400 mL

 

Fix for Antigen Staining

            (PBS, 10 % sucrose, 3.7 % formaldehyde)

            5 mL                10 X PBS

            5 g                   sucrose

            5 mL                37 % formaldehyde

           

            Add DI H2O to 50 mL

 

DAB for Westerns (30 mL)

            3 mL                10 % imidazol

            30 mg              DAB

            30 uL               30 % H2O2 (Hydrogen peroxide)

 

            Add DI H2O to 30 mL

 

1 M Tris (pH 7.4     500 mL)

            60.5 g              Tris Base

            350 mL            DI H2O

            pH with HCl to pH 7.4 (~50 mL)

           

            Add DI H2O to 500 mL

 

Completed Lysis Buffer for PI3K (100 mL)

            4.57 mL           3 M NaCl                    (137 mM NaCl)

            2 mL                1 M Tris (pH 7.5)        (20 mM Tris)

            0.1 mL             1 M MgCl2                 (1 mM MgCl2)

            0.1 mL             1 M CaCl2                  (1 mM CaCl2)

            10 mL              100 % glycerol            (10 % glycerol)

            1 mL                NP-40                          (1 % NP-40)

 

            Add DI H2O to 100 mL

            Store at 4C.

            Just before use, add protease inhibitors.

 

 

 

 

 

 

 

Poly-lysine – Laminin Coating for cover slips

            10 mL              poly-lysine

            79 uL               laminin

            3.78 mL           Sterile DI H2O

 

            100 – 150 uL per cover slip (12 mm round)

            1 hr at RT

            Remove poly-lysine/laminin

            Wash 2 X with Sterile DI H2O

            Leave wet until use

 

 1 X Semi-Dry Western Transfer Buffer

            2.93 g              Glycine                        (39 mM Glycine)

            5.81 g              Tris-Base                     (48 mM Tris)

            0.375 g            SDS

            100 mL            DI H2O

            200 mL            Methanol

           

            Add DI H2O to 1 L

 

Mono Q buffers

            QA (2 L: 20 mM Tris pH 7.7, 100 mM KCl, 1 mM MgCl2, 1 mM DTT)

            40 mL              1 M Tris (pH 7.7)

            14.9 g              KCl

            0.406 g            MgCl2

            2 mL                1 M DTT         (1.54g DTT in 10 mL DI H2O = 1 M DTT)

 

            Add DI H2O to 2 L

 

            QA (alt)

            1 X XB Salts (20 X Stock)

            20 mM Tris-HCl (pH 7.7)

           

            Degas before using on FPLC

 

            QB (1 L QA + 0.9 M KCl)

            1 L                   QA

            67.1 g              KCl

 

            QB (alt)

            1 L                   QA (alt)

            141.6 g            KCl                 (1.9 M KCl)

           

            Degas before using on FPLC

 

 

TAE Buffer (50 X stock     1 L)

            242 g               Tris Base

            57.1 mL           Glacial Acetic Acid

            100 mL            0.5 M EDTA (pH 8.0)

 

            Add DI H2O to 1 L

 

PBS (1 X     100 mL)

            0.8 g                NaCl

            0.2 g                KCl

            0.115 g            Na2HPO4

            0.02 g              KH2PO4         pH ~7.1-7.2

 

            Filter Sterilize

 

Carbenicilin (100 X)

            10 mg / mL      Carbenicilin

 

            Filter sterilize, then freeze/store 1 mL aliquots

 

20 X SSC

            (3 M NaCl, 0.3 M Na3Citrate)

            175.3 g            NaCl

            88.2 g              Na3Citrate

            800 mL            DIH2O

 

            Adjust pH to 7.0 with 1 M HCl

            Add DI H2O to 1 L

 

Kanamycin (1000 X)

            30 mg / mL      Kanamycin

           

            Filter sterilize, then freeze/store 1 mL aliquots

            1000 X stock for pET vectors

            600 X for BactoBAC cells

 

IPTG (1 M Stock)

            2.4 g                IPTG

            10 mL              DI H2O

           

            Filter sterilize, then freeze/store 1 mL aliquots

            0.4 - 1.0 mM final working concentration (Stock is 1000 – 2500 X)

 

Lysis Buffer for sf9/baculo cells

            20 mL              1 X TBS

            20 uL               Triton X-100   (0.1 % Triton X-100)

 

2 X Sample Buffer (10 mL)

            1 mL                1 M Tris (pH 6.8)

            2 mL                20 % SDS

            2 mL                100 % Glycerol

            2 mL                1 % Bromophenol Blue

            3 mL                DI H2O

 

            Add 0.2 mL 1 M DTT prior to use

 

Buffer for S300 Column (1 L     for CDC16)

            20 mL              1 M Tris (pH 7.7)

            7.45 g              KCl

            1 mL                1 M DTT

 

            Optional: add 1 mL of 1 M MgCl2

            Add DI H2O to 1 L

 

Stacking Gel Solution

            8 mL                Acrylamide:bis 37.5:1

            7.5 mL             1 M Tris (pH 6.8)

            0.3 mL             20 % SDS

            44.4 mL           DI H2O

 

            Makes 50 mL to be stored at 4C

            For each 5 mL (one gel): add 50 uL 10 % APS (ammonium persulfate)

                        and 5 uL TEMED

 

Western Strip Buffer (100 mL)

            6.25 mL           1 M Tris (pH 6.7)

            10 mL              20 % SDS

            0.7 mL             BME (beta-Mercapto Ethanol)

 

            Add DI H2O to 100 mL

            Wash Blot 30 min at 50C

 

0.5 M Imidazole for bead elution (100 mL)

            3.4 g                Imidazole

            1 mL                0.5 M NaHPO4 (pH 6)

            3.3 mL             1 M KCl

           

            Add DI H2O to 100 mL

 

0.5 M NaHPO4 (pH 6     30 mL)

            20 mL              0.5 M NaH2PO4 (pH  ~4.3)

            10 mL              0.5 M Na2HPO4 (pH ~7.2)

 

0.5 M NaH2PO4 (100 mL     mw 120)

             6 g                  NaH2PO4

            100 mL            DI H2O

 

0.5 M Na2HPO4 (100 ml     mw 142)

            7.1 g                Na2HPO4

            100 mL            DI H2O

 

1 M DTT (10 mL Stock     fw 154.2)

            1.542 g            DTT

            10 mL              DI H2O

 

            Aliquot and store at -20C

 

20 X PBS (500 mL)

            80 g                 NaCl

            20 g                 KCl

            11.5 g              Na2HPO4

            2 g                   KH2PO4 (pH 7.1-7.2)

 

            Add DI H2O to 500 mL and filter sterilize

 

4 % paraformaldehyde (100 mL)

            4 g                   paraformaldehyde

            100 mL            hot (60 – 65C) DI H2O

 

100 mM KxFe(CN)6 (100 mL)

            4.2 g                K4Fe(CN)6

            3.3 g                K3Fe(CN)6

            100 uL             1 M MgCl2

            100 mL            1 X PBS

 

6M Gu-HCl, 0.1 M Na Phosphate, 0.01 M Tris-HCl (pH 8) (50 mL)

            28.7 g              GuHCl (fw 95.53)

            10 mL              0.5 M NaHPO4 (pH 7.2)

            0.5 mL             1 M Tris (pH 8.0)

 

            Add DI H2O to 50 mL

 

 

 

 

 

 

 

Dialysis Buffer for APC urea Dialysis (1.5 L)

            15 mL              1 M Tris (pH 7.4)        (10 mM Tris, pH 7.4)

            26.5 g              NaCl                            (300 mM NaCl)

            0.3 g                MgCl2                         (1 mM MgCl2)

            1.5 mL             1 M DTT                     (1 mM DTT)

            3 mL                0.5 M EDTA               (1 mM EDTA)

            0.22 g              CaCl2                          (1 mM CaCl2)

            0.15 g              BSA                            (0.1 mg/mL BSA)

           

180 g               Urea                            (2 M Urea)

                                    or

            90 g                 Urea                            (1 M Urea)

 

XB Extraction Buffer (20 X     1 X)

            20 X Salts (2 M KCl, 20 mM MgCl2, 2 mM CaCl2)

            1 X Salts (100 mM KCl, 1 mM MgCl2, 0.1 mM CaCl2)

 

            1 X (1 X Salts, 10 mM Potassium HEPES (pH 7.7), 50 mM Sucrose)

 

            20 X XB Salts Buffer (500 mL)

            74.55 g            KCl

            0.147 g            CaCl2-2H2O

            2 g                   MgCl2

 

            Add DI H2O to 500 mL

 

PIN-1 Wash Buffer (20 mL)

            1 mL                1 M Tris (pH 8.0)

            2 mL                2 M NaCl

            4 mL                0.5 M NaF

            2 mL                100 % Glycerol

            0.2 mL             Triton X-100

            0.4 mL             0.5 M EDTA (pH 8.0)

            1 uM                microcystin (okadaic acid)

            1 X                  protease inhibitors

 

Cyclohexamide (100 X Stock)

            10 mg / mL      Cyclohexamide in DI H2O

           

            Store frozen aliquots at -80C

 

 

 

 

 

 

Energy Mix (20 X     5 mL)

            0.245 g            Creatine Phosphate

            1 mL                100 mM ATP

            100 uL             1 M MgCl2

           

            Add DI H2O to 5 mL

            Store in aliquots at -20C

 

EB (1 X     400 mL)

            6.912 g            glycerophosphate        (80 mM)

            3.044 g            EGTA                         (20 mM)

            1.22 g              MgCl2                         (15 mM)

            300 mL            DI H2O

 

            pH to 7.8 with KOH

            Add DI H2O to 400 mL

 

Sperm Dilution Buffer (1 x     5 mL)

            5 uL                 1 M MgCl2                 (1 mM MgCl2)

            0.5 mL             1 M KCl                      (100 mM KCl)

            375 uL             2 M Sucrose                (150 mM Sucrose)

            25 uL               1 M HEPES (pH 7.7)  (5 mM HEPES)

            4.1 mL             DI H2O

 

Fix for Nuclei (make fresh)

            0.3 vol             37 % formaldehyde

            0.6 vol             80 % w/v glycerol

            0.1 vol             10 X MMR

            1 ug / mL         Hoechst dye bis benzimide     (10 mg / mL stock at -20C)

 

CSF-XB (100 mL)

            5 mL                20 X XB Salts             (1 X Salts)

            1 mL                1 M HEPES                (10 mM HEPES)

            2.5 mL             2 M Sucrose                (50 mM Sucrose)

            1 mL                0.5 M EGTA               (5 mM EGTA)

 

2 X – XN Buffer (100 mL)

            10 mL              1 M HEPES (KOH, pH 7.0)   (100 mM HEPES)

            10 mL              2 M Sucrose                            (200 mM Sucrose)

            7.5 mL             2 M NaCl                                (150 mM NaCl)

            25.4 mg           Spermidine-4HCl                    (1 mM Spermidine)

            10.44 mg         Spermine-4HCl                       (0.3 mM Spermine)

 

            Add DI H2O to 100 mL

 

 

G-PEM Buffer for rho tubulin

            80 mM             PIPES (pH 6.8)

            1 mM               MgCl2

            1 mM               EGTA

            1 mM               GTP

            10 %                glycerol

 

0.5 M EGTA (100 mL     pH 7.7)

            19 g                 EGTA

            70 mL              DI H2O

           

            pH to 7.7 with 10 N NaOH (~10-15 mL)

            Add DI H2O to 100 mL

 

25 X MMR (Marc’s Modified Ringers     2 L     pH 7.8)

            292.2 g            NaCl

            7.45 g              KCl

            10.15 g            MgCl2

            14.7 g              CaCl2

            1.8 g                EDTA

            59.6 g              HEPES

            1.2 L                DI H2O

 

            pH to 7.8 with NaOH

            Add DI H2O to 2 L

 

Nuclei Isolation Buffer A (100 mL)

            (60 mM KCl, 15 mM NaCl, 0.15 mM Spermine, 0.5 mM Spermidine,

            15 mM Tris (pH 7.4), 0.2 mM EDTA, 0.2 mM EGTA)

           

            6 mL                1 M KCl

            1.5 mL             1 M Tris (pH 7.4)

            0.5 mL             3 M NaCl

            40 uL               0.5 M EDTA

            40 uL               0.5 M EGTA

            150 uL             100 mM Spermine

            250 uL             200 mM Spermidine

 

0.5 M Na pyrophosphate (dibasic) (20 mL     pH 7.0) 

            2.21 g / 20 mL

 

            pH to 7.0 with phosphoric acid

 

 

 

 

Stop Buffer (or Stabilization Buffer) SB (10 mL     pH 7.0)

            (50 mM NaF, 40 mM beta-glycerophosphate, 10 mM EDTA,

10 mM Na Pyrophosphate pH 7.0)

 

1 mL                0.5 M NaF

0.4 mL             1 M beta-glycerophosphate

0.2 mL             0.5 M EDTA

0.2 mL             0.5 M Na2HP2O7

 

2 X YT (per liter)

            16 g                 bactotryptone

            10 g                 bacto yeast

            5 g                   NaCl

           

            pH to 7.0 with 5 N NaOH

 

Wash Buffer for GST Purification (200 mL     pH 8.2)

            10 mL              1 M Tris (pH 8.2)        (50 mM Tris)

            2.96 g              KCl                             (200 mM)

            0.2 mL             1 M DTT                     (1 mM DTT)

           

            Add protease inhibitors before use

 

Lysis Buffer for GST Purification

            Wash Buffer for GST Purification + 0.3 mM PMSF

            + 200 ug / mL lysozyme

 

10x Taq/Pfu Buffer

(from Teresita/Ethan August 2003)

 

200 mM  Tris HCL

20   mM  MgSO4

100 mM  KCl

100 mM  (NH4)2 SO4

 

1 mg/ml Nuclease-free BSA

 

FILTER
-------------------------------------

1% Triton X-100

 

 

 

 

AMINO ACIDS

(from Teresita/Ethan September 2001)

 

Amino Acid Mix (minus Methionine, Cysteine, Cystine)

 

  • Assume 110g/ mole for each amino acid.
  • Want to add enough to make 2mM stock

 

110g/ X = 1 M/ 0.002M (= 2 mM)

 

therefore X= 220 mg/ liter

 

  • To make 50 ml
    • Add 11 mg of each amino acid EXCEPT: METHIONINE, CYSTEINE, CYSTINE
    • Bring to 40 ml with sterile water, pH to 7.0 with either HCL or NaOH add water to 50 ml in a sterile beaker/tube.

2 mM Methionine

            11 mg Methionine

            40 ml H20

            pH to 7.0

            add H20 to 50 ml

 

2 mM Cysteine/ Cystine

            11 mg Cysteine

            11 mg Cystine

            40 ml H20

pH to 7.0

H20 to 50 ml

 

L-Amino Acids (SIGMA LAA-21) – 1 Kit

 


Translation Reaction Mix

(Teresita/Ethan May 2002)

 

Promega

 

Per Reaction (5 μl)

 

TnT Retic lysate                              2.5 μl

Tnt Reaction buffer                         0.2 μl

RNA pol. SP6                                 0.1 μl

AA (minus Met)                              0.1 μl

S35 Methionine                                0.4 μl

Rnasin                                             0.1 μl

H20                                                 1.6 μl

                                                                                      

                                                         5.0μl

 

 

 

 

IVT

 

1)      5 μl TnT Mix + 1 μl DNA

2)       Incubate 1 hour @ 30 0C

 

 

 

 

 

Homemade (5X E/T Buffer)

 

Per Reaction (5 μl)

 

Retic lysate*                                   2.5 μl

5X Reaction buffer                            1 μl

RNA pol. SP6                                 0.1 μl

AA (minus Met/ Cys)                    0.1 μl

S35 (Met/Cys)                                 0.4 μl

Rnasin                                             0.1 μl

H20                                                  0.8 μl

                                                                                      

                                                         5.0μl

 

*Promega Catalog # L4960

 

 

Enhanced Chemiluminescence (ECL)2

(Teresita/ Licio/ Ethan May 2002)

 

 

Stock Solution:

 

250 mM Luminol (in DMSO)

90 mM p-Coumaric acid in (DMSO)

30% Hydrogen peroxide (H202)

100 mM Tris-HCL (pH 8.5-9.0)

DMSO

 

Solution A (Final concentration):

 

0.0165% H2O2

100 mM Tris-HCL

 

Volumes for 20 (final) ml of ECL Buffers

 

Stock Solutions

 

100mM Tris-HCL

30 % H2O2

250 mM Luminol

90 mM p-Coumaric Acid

 

FLUKA 09253        5 g

SIGMA C-9008     10 g

SIGMA H-1009     5 ml

 


SIGMA D-2650 100 ml

 

Solution B (Final concentration):

 

2.5 mM Luminol

0.4 mM p-Coumaric acid

100 mM Tris-HCl

 

Solution A

Solution B

10 ml

10 ml

5.5 μl

--------

---------

100 μl

---------

 45  μl

 

 


5X TnT Reaction Buffer

(Teresita/ Ethan May 2002)

 

 

 

Final Concentration:

 

50

mM

Potassium acetate

2

mM

Magnesium Chloride

0.05

mg/ml

Creatine kinase

1   

mM

Creatine phosphate

1

mM

ATP

1

mM

CTP

1

mM

GTP

1

mM

UTP

 

 

Stock Solution:

 

1

M

Potassium Acetate

------

 

40

mM

Magnesium Chloride

------

 

1

Mg/ml

Creatine Kinase

ROCHE 127 566

100 mg

100

mM

Creatine phosphate

ROCHE 621 714

5 g

100

mM

ATP

SIGMA A-9062

100 mg

100

mM

CTP

SIGMA C-1631

100 mg

100

mM

GTP

SIGMA G-9002

100 mg

100

mM

UTP

SIGMA U-6875

100 mg