Publications
Steen JA, Steen H, Georgi A, Parker K, Springer M, Kirchner M, Hamprecht F, Kirschner MW. Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6069-74. Epub 2008 Apr 17. PMID: 18420821 [PubMed - indexed for MEDLINE]
Milo R, Hou JH, Springer M, Brenner MP, Kirschner MW. The relationship between evolutionary and physiological variation in hemoglobin. Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):16998-7003. Epub 2007 Oct 17. PMID: 17942680 [PubMed - in process]
Rickmyre JL, Dasgupta S, Ooi DL, Keel J, Lee E, Kirschner MW, Waddell S, Lee LA. The Drosophila homolog of MCPH1, a human microcephaly gene, is required for genomic stability in the early embryo. J Cell Sci. 2007 Oct 15;120(Pt 20):3565-77. Epub 2007 Sep 25. PMID: 17895362 [PubMed - in process]
Weiner OD, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW. An Actin-Based Wave Generator Organizes Cell Motility. PLoS Biol. 2007 Sep;5(9):e221 PMID: 17696648 [PubMed - in process]
Reddy SK, Rape M, Margansky WA, Kirschner MW. Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation. Nature. 2007 Apr 19;446(7138):921-5. PMID: 17443186
Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, Elledge SJ. Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities. Nature. 2007 Apr 19;446(7138):876-81. PMID: 17443180
Wang R, He G, Nelman-Gonzalez M, Ashorn CL, Gallick GE, Stukenberg PT, Kirschner MW, Kuang J. Regulation of Cdc25C by ERK-MAP kinases during the G2/M transition. Cell. 2007 Mar 23;128(6):1119-32. PMID: 17382881
Kirschner MW. Obituary: Reinhart Heinrich (1946-2006). Nature. 2006 Dec 7;444(7120):700. No abstract available. PMID: 17151654
Bourlat SJ, Juliusdottir T, Lowe CJ, Freeman R, Aronowicz J, Kirschner M, Lander ES, Thorndyke M, Nakano H, Kohn AB, Heyland A, Moroz LL, Copley RR, Telford MJ. (2006) Deuterostome phylogeny reveals monophyletic chordates and the new phylum Xenoturbellida. Nature 444 (7115): 85-8. PMID: 17051155
Peterson JR, Lebensohn AM, Pelish HE and Kirschner MW (2006) Biochemical Suppression of Small-Molecule Inhibitors: A Strategy to Identify Inhibitor Targets and Signaling Pathway Components. Chemistry & Biology 13: 443-452.
Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. (2006) Hem-1 Complexes Are Essential for Rac Activation, Actin Polymerization, and Myosin Regulation during Neutrophil Chemotaxis. PLoS Biol. 4(2):e38 [Epub ahead of print] PMID: 16417406
Ho HYH, Rohatgi R, Lebensohn AM, and Kirschner MW (2006) In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components. Methods Enzymol. 406: 174-190.
Lebensohn AM, Ma L, Ho HYH, and Kirschner MW (2006)
Cdc42 and PI(4,5)P2-Induced Actin Assembly in Xenopus
Egg Extracts. Method Enzymol.
406: 156-173.
Rape M, Reddy SK, Kirschner MW. (2006) The processivity of multiubiquitination by the APC determines the order of substrate degradation. Cell 124(1):89-103.
Steen H, Jebanathirajah JA, Rush J, Morrice N, Kirschner MW. (2006) Phosphorylation analysis by mass spectrometry: Myths, facts and the consequences for qualitative and quantitative measurements. Mol Cell Proteomics 5.1:172-181. PMID: 16204703.
Ayad NG, Rankin S, Ooi D, Rape M, Kirschner MW. (2005) Identification of ubiquitin ligase substrates by in vitro expression cloning. Methods Enzymol. 399:404-14.
Jebanathirajah JA, Pittman JL, Thomson BA, Budnik BA, Kaur P, Rape M, Kirschner M, Costello CE, and O'Connor PB. (2005) Characterization of a New qQq-FTICR Mass Spectrometer for Post-Translational Modification Analysis and Top-Down Tandem Mass Spectrometry of Whole Proteins. J Am Soc Mass Spectrom. 16(12):1985-99.
Kwan, KM and Kirschner, MW (2005) A microtubule-binding Rho-GEF controls cell morphology during convergent extension of Xenopus laevis. Development 132(20):4599-610.
Viadiu, H., Stemmann, O., Kirschner, M.W., Walz, T. (2005) Domain structure of separase and its binding to securin as determined by EM. Nat Struct Mol Biol. 12 (6):552-3.
Gerhart, J., Lowe, C., Kirschner, M. (2005) Hemichordates and the origin of chordates. Current Opinion in Genetics and Development 15 (4): 461-467.
Kirschner, M.W. (2005) The Meaning of Systems Biology (commentary). Cell 121: 503-504.
Rankin S, Ayad NG, Kirschner MW. (2005) Sororin, a substrate of the anaphase- promoting complex, is required for sister chromatid cohesion in vertebrates. Mol Cell. 18(2):185-200.
Steen H, Jebanathirajah JA, Springer M, Kirschner MW. (2005) Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A. 102(11):3948-53.
Lee LA, Lee E, Anderson MA, Vardy L, Tahinci E, Ali SM, Kashevsky H, Benasutti M, Kirschner MW, Orr-Weaver TL. (2005) Drosophila genome-scale screen for PAN GU kinase substrates identifies Mat89Bb as a cell cycle regulator. Dev Cell. 8(3):435-42.
Lowe, C. J., Tagawa, K., Humphreys, T., Kirschner, M., Gerhart, J. (2004) Hemichordate embryos: procurement, culture, and basic methods. Methods Cell Biol. 74: 171-194.
Rape, M., and Kirschner, M. W. (2004) Autonomous regulation of the anaphase-promoting complex couples mitosis to S-phase entry. Nature. Dec 2; 432 (7017): 588-595. Epub 2004 Dec 2.
Margalit, D.N., Romberg, L., Mets, R.B., Hebert, A.M., Mitchison, T.J., Kirschner, M.W., RayChaudhuri, D. (2004) Targeting cell division: Small-molecule inhibitors of FtsZ GTPase perturb cytokinetic ring assembly and induce bacterial lethality. Proc. Natl. Acad. Sci. USA. 101(32): 11821-11826. Epub 2004 Aug 02.
Peterson, J.R., Bickford, L.C., Morgan, D., Kim, A.S., Ouerfelli, O., Kirschner, M.W., Rosen, M.K. (2004) Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat Struct Mol Biol. Aug; 11(8):747-755. Epub 2004 Jul 04.
Ho, H.Y., Rohatgi, R., Lebensohn, A.M., Ma, L., Li, J., Gygi, S.P., Kirschner, M.W. (2004) Toca-1 Mediates Cdc42-Dependent Actin Nucleation by Activating the N-WASP-WIP Complex. Cell 118 (2): 203-216.
Martinez-Quiles, N., Ho, H.Y., Kirschner, M.W., Ramesh, N., Geha, R.S. (2004) Erk/Src Phosphorylation of Cortactin Acts as a Switch On-Switch Off Mechanism That Controls Its Ability To Activate N-WASP. Mol Cell Biol. 24(12): 5269-5280.
Gautreau, A., Ho, H.Y., Li , J., Steen, H., Gygi , S.P., Kirschner, M.W. (2004) Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A. 101(13): 4379-83.
Wei ,W., Ayad, N.G., Wan, Y., Zhang, G.J., Kirschner, M.W., Kaelin, W.G., Jr. (2004) Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex. Nature 428 (6979): 194-8.
Campellone, K.G., Rankin, S., Pawson, T., Kirschner, M.W., Tipper, D.J., and Leong, J.M. (2004) Clustering of Nck by a 12- residue Tir phosphopeptide is sufficient to trigger localized actin assembly. Journal ofCell Biology 164 (3): 407416.
Song, M., Song, S., Ayad, N., Lee, J., Hong, H., Chang, J., Kim, J., Choi, E., Kirschner, M., Lim, D. (2004) The tumor suppressor RASSF1A regulates mitotic progression through interaction with Cdc20 and inhibition of the anaphase promoting complex. Nature Cell Biol. 6(2):129-137.
Lee, E., Salic, A., Krger, Heinrich, R., Kirschner, M.W. (2003) The Roles of APC and Axin Derived from Experimental and Theoretical Analysis of the Wnt Pathway. Public Library of Science 1: 116-132.
Lowe, C.J., Wu, M., Salic, A., Evans, L., Lander, E., Stange-Thomann, N., Gruber, C.E., Gerhart, J., and Kirschner, M. (2003) Anteroposterior Patterning in Hemichordates and the Origins of the Chordate Nervous System. Cell 113: 853-865.
Ayad, N.G., Rankin, S., Murakami, M., Jebanathirajah, J., Gygi, S., and Kirschner, M.W. (2003) Tome-1, a Trigger of Mitotic Entry, is Degraded During G1 via the APC. Cell 113: 101-113 .
Kwan, K.M. and Kirschner, M.W. (2003). Xbra functions as a switch between cell migration and convergent extension in the Xenopus gastrula. Development 130: 1961-1972.
Eden, S., Rohatgi, R., Podtelejnikov, A.V., Mann, M., and Kirschner, M.W. (2002). Mechanism of regulation of WAVE1-inducedactin nucleation by Rac1 and Nck. Nature418: 790-793.
Georgi, A.B., Stukenberg, P. T., and Kirschner, M.W. (2002). Timing of Events in Mitosis. Current Biology 12: 105-114.
Stemmann, O., Zou, H., Gerber, S.A., Gygi, S.P., and Kirschner, M.W. (2001). Dual Inhibition of Sister Chromatid Separation at Metaphase. Cell 107: 715-726.
Wan, Y., Liu, X., and Kirschner, M.W. (2001). The Anaphase-Promoting Complex Mediates TGF-β Signaling by Targeting SnoN for Destruction. Molecular Cell 8: 1027-1039.
Wan, Y. and Kirschner, M.W. (2001). Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities. Proc. Natl. Acad. Sci. USA 98: 13066-13071.
Davis, R.L., Turner, D.L., Evans, L.M., and Kirschner, M.W. (2001). Molecular Targets of Vertebrate Segmentation: Two Mechanisms Control Segmental Expression of Xenopus hairy2 during Somite Formation. Developmental Cell 1: 553-565.
Ho, H.-Y., Rohatgi, R., Ma, L., and Kirschner, M.W. (2001). CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family. Proc. Natl. Acad. Sci. USA 98: 11306-11311.
Peterson, J.R., Lokey, R.S., Mitchison, T.J., and Kirschner, M.W. (2001). A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions. Proc. Natl. Acad. Sci. USA 98: 10624-10629.
Lee, E., Salic, A., and Kirschner, M.W. (2001). Physiological regulation of β-catenin stability by Tcf3 and CK1ε. Journal of Cell Biology 154: 983-993.
Pfleger, C.M., Lee, E., and Kirschner, M.W. (2001). Substrate recognition by the Cdc20 and Cdh1 components of the Anaphase Promoting Complex. Genes & Development, 15:2396-407.
Rohatgi, R., Nollau, P.; Ho, H., Kirschner, M.W., and Mayer, B.J. (2001) Nck and Phosphatidylinositol 4,5-Bisphosphate Synergistically Activate Actin Polymerization through the N-WASP-Arp2/3 Pathway. Journal of Biological Chemistry 276: 26448-26452.
Pfleger, C.M., Salic, A., Lee, E., and Kirschner, M.W. (2001) Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1. Genes & Development 15:1759-1764.
Stukenberg, P.T. and Kirschner, M.W. (2001) Pin1 Acts Catalytically to Promote a Conformational Change in Cdc25. Molecular Cell 7: 1071-1083.
Yamaguchi, H., Miki, H., Suetsugu, S., Ma, L., Kirschner, M.W., and Takenawa, T. (2000) Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP. Proc. Natl. Acad. Sci. USA 97: 12631-12636.
Rohatgi, R., Ho, H., and Kirschner, M.W. (2000) Mechanism of N-WASP Activation by CDC42 and Phosphatidylinositol 4,5-bisphosphate. Journal of Cell Biology 150 (6): 1299-1309.
Chen, F., Ma, L., Parrini, M.C., Mao, X., Lopez, M., Wu, C., Marks, P.W., Davidson, L., Kwiatkowski, D.J., Kirchhausen, T., Orkin, S.H., Rosen, F.S., Mayer, B.J., Kirschner, M.W., and Alt, F.W. (2000) Cdc42 is required for PIP (2)-induced actin polymerization and early development but not for cell viability. Current Biology 10 (13): 758-765.
Luo, X., Fang, G., Coldiron, M., Lin, Y., Yu, H., Kirschner, M.W., and Wagner, G. (2000) Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Nature Structural Biology 7: 224-229.
Pfleger, C.M. and Kirschner, M. W. (2000) The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1. Genes & Development 14: 655-665.
Salic, A., Lee, E., Mayer, L., and Kirschner, M.W. (2000) Control of b-Catenin Stability: Reconstitution of the Cytoplasmic Steps of the Wnt Pathway in Xenopus Egg Extracts. Molecular Cell 5: 523-532.
Davis, R.L. and Kirschner, M.W. (2000) The fate of cells in the tailbud of Xenopus laevis. Development 127: 255-267.
Kroll, K.L. and Kirschner, M.W. (1999) Easy passage: Germline transgenesis in frogs (Commentary). Proc. Natl. Acad. Sci. USA 96: 14189-14190.
Fang, G., Yu, H., and Kirschner, M.W. (1999) Control of mitotic transitions by the anaphase-promoting complex. Phil. Trans. R. Soc. Lond. 354: 1583-1590.
Odde, D.J., Ma, L., Briggs, A.H., DeMarco, A., and Kirschner, M.W. (1999) Microtubule Bending and Breaking in Living Fibroblasts. Journal of Cell Science 112: 3283-3288.
Zou, H., McGarry, T.J., Bernal, T., and Kirschner, M.W. (1999) Identification of a Vertebrate Sister-Chromatid Separation Inhibitor Involved in Transformation and Tumorigenesis. Science 285: 418-422.
Rohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., and Kirschner, M.W. (1999) The Interaction between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly. Cell 97: 221-231.
Zhou, B. and Kirschner, M.W. (1999) Quantitative Measurement of the Catastrophe Rate of Dynamic Microtubules. Cell Motility and the Cytoskeleton, 43: 43-51.
Philpott, A., Tsai, L., and Kirschner, M.W. (1999) Neuronal Differentiation and Patterning in Xenopus: The Role of cdk5 and a Novel Activator Xp35.2. Developmental Biology 207: 119-132.
Haushalter, K.A., Stukenberg, P.T., Kirschner, M.W. and Verdine, G.L. (1999). Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors. Current Biology 9: 174-185.
Zou, H., McGarry, T.J., Bernal, T., and Kirschner, M.W. (1999) Identification of a Vertebrate Sister-Chromatid Separation Inhibitor Involved in Transformation and Tumorigenesis. Science 285: 418-422.
Rohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., and Kirschner, M.W. (1999) The Interaction between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly. Cell 97: 221-231.
Zhou, B. and Kirschner, M.W. (1999) Quantitative Measurement of the Catastrophe Rate of Dynamic Microtubules. Cell Motility and the Cytoskeleton, 43: 43-51.
Ma, L., Rohatgi, R. and Kirschner, M.W. (1998). The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42. Proc. Natl. Acad. Sci. USA 95: 15362-15367.
Fang, G., Yu, H., and Kirschner, M.W. (1998). Direct Binding of CDC20 Protein Family Members Activates the Anaphase-Promoting Complex in Mitosis and G1. Mol. Cell 2: 163-171.
Kirschner, M. and Gerhart, J. (1998). Evolvability (Perspective). Proc. Natl. Acad. Sci. USA 95: 8420-8427.
McGarry, T.J. and Kirschner, M.W. (1998). Geminin: An Inhibitor of DNA Replication is Degraded during Mitosis. Cell 93: 1043-1053.
Fang, G., Yu, H., and Kirschner, M.W. (1998). The Checkpoint Protein MAD2 and the Mitotic Regulator CDC20 Form a Tenary Complex with the Anaphase-Promoting Complex to Control Anaphase Initiation. Genes & Development 12: 1871-1883.
Kroll, K.L., Salic, A., Evans, L., and Kirschner, M.W. (1998). Geminin, a neuralizing molecule that demarcates the future neural plate at the onset of gastrulation. Development 125: 3247-3258.
Ma, L., Cantley, L.C., Janmey, P.A., and Kirschner, M.W. (1998). Co-requirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts. Journal of Cell Biology 140: 1125-1136.
Yu, H., Peters, J-M., King, R.W., Page, A., Hieter, P., and Kirschner, M.W. (1998). Identification of a Cullin Homology Region in a Subunit of the Anaphase-Promoting Complex [AU1]. Science 279: 1219-1222.
Kirschner, M. and Gerhart, J. (1998). Comment on "Epigenetic inheritance in evolution". [Commentary] Journal of Evolutionary Biology 11: 213-217.
Lustig KD, Stukenberg PT, McGarry TJ, King RW, Cryns VL, Mead PE, Zon LI, Yuan J and Kirschner MW. (1997) Small Pool Expression Screening: A Novel Strategy for the Identification of Genes Involved in Cell Cycle Control, Apoptosis and Early Development. Methods in Enzymology 283: 83-99. PMID: 9251013
Murray AW and Kirschner MW. (1989) Cyclin synthesis drives the early embryonic cell cyle. Nature 339 (6222): 275-280. PMID: 2566917
Cyert MS and Kirschner MW. (1988) Regulation of MPF activity in vitro. Cell 53 (2): 185-195. PMID: 2834064
Books
Gerhart, J.C. and Kirschner, M.W. Cells, Embryos and Evolution. Boston: Blackwell Science, 1997.
Kirschner, M.W. and Gerhart, J.C. The Plausibility of Life: Resolving Darwin’s Dilemma. New Haven: Yale University Press, 2005.